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Aggregate analysis Product List

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Analysis of protein aggregates by size exclusion chromatography (SEC)

Evaluation by SEC is effective for protein quality control!

Quality control of proteins, including biopharmaceuticals, is important, particularly regarding aggregates such as dimers and oligomers, as well as degradation products (low molecular weight species), which have gained attention in recent years due to their relationship with immunogenicity. Size exclusion chromatography (SEC) is widely used as a simple method for analyzing these. Here, we present examples of analyses conducted on commercially available protein mixtures, IgG antibodies, and IgG antibodies that were aggregated through pH stress testing. *For more details, please refer to the PDF document or feel free to contact us.

  • Contract measurement
  • Contract Inspection

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Analysis of multivalent aggregates of monoclonal antibodies using size exclusion UPLC.

Improvement of separation of high molecular weight proteins up to 1.8 MDa.

We will demonstrate the advancements in size exclusion separation based on UPLC using data analyzed from polymer protein complexes, comparing it with HPLC data. Additionally, we will analyze monoclonal antibodies containing multivalent aggregates by connecting ACQUITY UPLC BEH SEC columns with pore sizes of 200 Å and 450 Å, showcasing the benefits obtained. 【Application Benefits】 ○ Compared to conventional HPLC-based SEC, improved separation of high molecular weight proteins up to 1.8 MDa. ○ In the analysis of monoclonal antibody aggregates containing dimers and higher-order multimers, the separation range is expanded by connecting the BEH450 SEC column with the BEH200 SEC column. For more details, please contact us or download the catalog.

  • Other laboratory equipment and containers

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